Th-p2a-egfp
WebApr 12, 2024 · For the p2A-EGFP (+HAs) donor, homologous arms were designed both upstream and downstream of the termination codon TAA site (Figure 1A). The 800-bp left … WebSep 2, 2024 · For the P2A–EGFP fragments in these constructs, we used BPK4335 (pCMV-BE3-P2A-EGFP) as a template. APOBEC3A constructs were cloned using JMG5377 (pCAG-hA3A-BE3) as a template. hAID-BE3 was ...
Th-p2a-egfp
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WebJul 1, 2015 · The adding of the right arm in donor plasmid - the 3′ intergenic region of targeted gene – is critically important. However, if a SV40 poly-A sequence rather than the 3′ intergenic sequence of the endogenous gene is used, no fluorescence can be detected in th-P2A-EGFP knockin and gfap-P2A-EGFP knockin (unpublished data WebFeb 5, 2024 · The continued expansion of the genome-editing toolbox necessitates methods to characterize important properties of CRISPR–Cas enzymes. One such property is the requirement for Cas proteins to ...
WebWe welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as … Webth-P2A-EGFP donor. catalog : 65562. more info or order : Addgene product webpage. citations: 1. Reference; Li J, Zhang B, Ren Y, Gu S, Xiang Y, Du J. Intron targeting-mediated …
WebThe th-P2A-EGFP cassette was integrated into the th locus after co-injection of the donor with the sgRNA and zCas9 mRNA. The zebrafish th has 13 exons, and E12 and E13 … WebOct 6, 2016 · A homologous recombination donor plasmid was designed to incorporate the P2A-EGFP cassette just upstream of the stop codon and with the puromycin selection gene downstream. FOXA2-2A-EGFP targeting donor vector, gRNA, and Cas9 expressing vectors were coelectroporated into hESCs and puromycin-resistant colonies were selected.
WebHover: Reveals additional information for enzyme cut sites, ORFs, feature descriptions, and primer details. Select: Click one location on the plasmid and then click on a second …
WebTools. Data Hub. Learn more about Addgene materials from user-contributed reports describing AAV and antibody experiments. Sequence Analyzer. Basic analysis for a user-entered sequence; includes restriction sites and map dunn chapel church of christ lufkin txWebFull Sequences from Depositor (1) Sequence provided by depositing laboratory may be theoretical/predicted or based on Sanger/NGS sequencing results. Discrepancies between … dunn chiropractic nashville tnWebZeocin® is an InvivoGen trademark. These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were … dunn city codeWebNov 5, 2015 · Fundamental and applied studies of silkworms have entered the functional genomics era. Here, we report a multi-gene expression system (MGES) based on 2A self … dunn coffee machineWebth-p2a-egfp应用于研究领域:发育生物学,工具鼠,荧光蛋白标记小鼠,神经系统,视力/眼睛,消化系统,肝脏/胆道系统,生理系统,行为 ... dunn coffee shopWebApr 7, 2015 · The th-P2A-EGFP cassette was integrated into the th locus after co-injection of the donor with the sgRNA and zCas9 mRNA. The zebrafish th has 13 exons, and E12 and … dunn coffey \\u0026 kasperitis p.cWebDescription. This AAV9 expresses functional gene eNpHR3.0 labeled by EGFP and driven by a hSyn promoter. Tracer Type. Virus Vector/Particals. Functional Gene. eNpHR3.0. Activity. Inhibition. Serotype. dunn coffey \u0026 kasperitis p.c