WebRemove lid from the plate. Apply the plate’s surface directly to the surface being tested and exert moderate pressure. Do not rub the agar surface or move laterally on the test surface! Return the lid and lock the plate by simply twisting the base and the lid of the plate into the locked position. Plates can be easily unlocked by untwisting. Dry the plates in the laminar flow hood with the lid slightly off for 30 minutes (or in a 37°C incubator for 2–3 hours, or room temperature for 2–3 days). Drying the plate is very important for storing the plates and growing colonies on them. If you don’t dry the plates, the moisture will evaporate and condense on the lid … See more Make up the medium according to the recipe, then add the desired amount of agar powder (normally about 1% w/v) and stir. If you autoclave without stirring, with the agarose still floating on top of the liquid, you get an … See more Autoclave your medium for 25 minutes. After autoclaving, you can, of course, store the medium-agar mix in a toughened glass bottle then melt it in a microwave or water bath when needed. … See more You can now add any antibiotics or supplements, and be confident that the agar is at a suitable temperature because you have cooled it in the water bath. See more Cool the medium-agar mix to 55°C. For routinely consistent results, do the cooling for a couple of hours in a 55°C water bath. Agar starts to solidify … See more
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WebJul 22, 2015 · after pouring the agar plates, leave the lid slightly open in a sterile hood till the plates cool down. It may take about a hour to be safe. Then leave put the lids fully and leave it at... WebThe updraft and heat of the flame will dry fairly moist plates out in 15-20 minutes. The most I've done is maybe 10 plates because you have to keep them in some proximity to the … the easiest quiz game cat
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WebDec 21, 2005 · If you really feel the need to let the condensation dry a bit, put your plates out (keep the lids on) and just leave them alone in a clean, cool place for a few hours. … WebIt is possible the plates are poured with too thin a layer of agar. Another option would be to incubate the plated in a chamber with some moist paper towels to provide a little … http://www.protocol-online.org/biology-forums-2/posts/10481.html the easiest minecraft seed